As a leading supplier in the field of microarrays, Creative Proteomics is committed to designing and developing various effective biological microarray products for non-clinical research and providing corresponding testing services. Our microarray test platform provides a full range of microarray-based protein analysis and determination services for inflammatory mediators to help our global customers conduct relevant research and reduce research costs. We are confident to provide you with high-quality products and high-quality services to help scientific research.
Peptide Epitope Mapping
The immune system has a rich library of antibodies that can target almost any molecular structure. The identification of antibody-targeted epitopes (B cell epitopes) is critical to the development of many diagnostic and therapeutic tools. As specific affinity reagents, antibodies have become indispensable tools for a wide range of scientific and diagnostic applications. For clinical use, these epitopes must be extensively characterized to verify specificity and document potential cross-reactivity. The efficiency, accuracy, and safety of these antibody-mediated applications mainly depend on the antibody selected against the expected rather than against any unexpected target structure. Therefore, specificity, that is, the typical characteristics of an antibody, not only has scientific significance but also has considerable practical significance. The epitope targeted by the antibody should be identified and characterized, thereby establishing antibody reactivity, highlighting possible cross-reactivity, and possibly even warn of non-essential (autoimmune) reactivity. An important aspect of verifying antibody specificity is to determine the structure of the epitope that the antibody interacts with. Epitope mapping has become a crucial link.
Service Process
We provide comprehensive epitope location services based on addressing protein microarray technology. Just send us your protein/antibody samples, and we will perform all the necessary processes from sample analysis to data analysis. The mapping resolution of linear epitopes depends on the length of the peptide, the overlap selected for the initial epitope, and the scale of subsequent fine specificity analysis. This includes N- and C-terminal truncation, amino acid scanning, random single, double or triple substitutions, and so on. Our comprehensive epitope location services include sequence design, custom-designed synthetic peptide microarrays for epitope maps; experimental quality control, microarray hybridization, array image scanning and data extraction; data analysis. Our array services include basic data analysis (fluorescence background reduction, data aggregation and processing, and report formation).
Fig 1. Epitope mapping microarray service process.
Our Features
- Comprehensive epitope location service
- Combination of high-throughput capacity and quantitative measurement
- Quickly and effectively identify high-affinity and high-specific target-binding compounds
- Strong bioinformatics team, perfect technology platform
Creative Proteomics provides high-quality microarray tools and convenient detection services for studying the inflammatory protein profile of inflammation-related signals. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. By choosing outsourcing testing services, your advantage is that you do not need to consider any other expenses, such as equipment, laboratory space, supervision or management. This convenient method can quickly turn around and provide reliable results, thus saving valuable time and resources. Please contact us for more detailed information.
References
- Hansen C S, Østerbye T, Marcatili P, et al. ArrayPitope: automated analysis of amino acid substitutions for peptide microarray-based antibody epitope mapping[J]. PloS one, 2017, 12(1): e0168453.
- Henkel S, Wellhausen R, Woitalla D, et al. Epitope mapping using peptide microarray in autoantibody profiling[M]//Microarray Technology. Humana Press, New York, NY, 2016: 209-224.
For research use only, not intended for any clinical use.
