As a trusted supplier in the field of microarrays, Creative Proteomics is committed to designing and developing various effective biological microarray products and providing corresponding testing services. Our microarray detection platform provides a full range of microarray-based analysis services to help our customers reduce costs while using advanced technologies for related research. We are confident to provide you with high-quality products and high-quality services to accelerate the output of research results.
Immunoassay and Microarray
In the diagnosis of complex diseases such as cancer, a set of biomarkers containing multiple proteins can provide higher sensitivity and specificity than a single biomarker. And this feature is that the antibody microarray is limited by the characteristics of the antibody itself, which has certain limitations in the analysis experiment. In order to improve this situation, the antibody colocalization microarray technology came into being. Antibody Co-localization Microarray (ACM), a variant of antibody microarray, is to avoid cross-reaction caused by reagents by printing a single detection antibody on the corresponding capture antibody. Eliminate unwanted interactions by running a single-plex detection array that is achieved by isolating the detection antibody in a single nanodroplet. When detecting proteins in biological fluids, the absence of cross-reactions can achieve a higher level of multiplex analysis, reduce background, improve sensitivity, and ensure accurate and specific results.
Fig 1. Schematic process flow for three sandwich assay formats, namely the ELISA (a singleplex assay), multiplexed sandwich assays (MSA) in microarray format, and the antibody colocalization microarray (ACM). (Pla-Roca M, et al. 2012)
Service Process
We provide multiple immunoassay microarray services based on co-localization microarray technology. The main content of this service includes combined immunoassays. Protein microarray-based array systems or suspension microarrays are recognized as a form of multiple sandwich immunoassay. In order to study inflammatory diseases, protein arrays can be used to analyze changes in plasma protein levels, such as cytokines, chemokines, soluble receptors and matrix metalloproteinases. Our service process includes microarray design and production, sample preparation, microarray hybridization, then spotting with detection antibodies, and finally harvesting data and conducting biological information analysis. The service process includes printing and incubation of capture antibody, incubation of diluted sample and antigen standard after washing and sealing. Then, after washing and drying, the homologous biotinylated detection antibody is printed at the same position as the captured antibody, incubated with fluorescent streptavidin, washed and dried, and then scanned and harvested. Choosing to outsource this application not only saves time and effort, but also benefits from technical expertise and facilities dedicated to antibody-based research. Such services can provide researchers with the advantages of various immunoassay options.
Fig 2. Service process of multiple immunoassays based on microarray.
Our Features
- High-throughput analysis of cytokine expression;
- Validation of semi-quantitative antibody array results;
- Identify potential molecular targets for drug development;
- Determine the molecular mechanism of drug action;
- Identify the key factors involved in the disease process;
- Discover biomarkers for disease management;
- Discover the expression patterns of molecular classification of diseases;
Creative Proteomics provides a high-quality microarray platform for multiple immunoassays. We have a large team of scientists with a solid academic foundation and cooperate with researchers in basic biology and biomedical projects from all over the world. The advantage of choosing an analytical testing outsourcing testing service is that there is no need to consider any other costs (such as equipment and laboratory space), and it saves supervision or management. This advanced technology can quickly turn around and provide reliable results, thereby saving valuable time and resources. Please contact us for more detailed information.
References
- Pla-Roca M, Leulmi R F, Tourekhanova S, et al. Antibody colocalization microarray: a scalable technology for multiplex protein analysis in complex samples[J]. Molecular & Cellular Proteomics, 2012, 11(4): M111. 011460.
- Laforte V, Lo P S, Li H, et al. Antibody colocalization microarray for cross-reactivity-free multiplexed protein analysis[M]//Serum/Plasma Proteomics. Humana Press, New York, NY, 2017: 239-261.
- Hsia C C, Chizhikov V E, Yang A X, et al. Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples[J]. Biochemical and biophysical research communications, 2007, 356(4): 1017-1023.
For research use only, not intended for any clinical use.
