As a leading supplier in the field of microarrays, Creative Proteomics is committed to designing and developing various effective biological microarray products for non-clinical research and providing corresponding testing services. Our microarray test platform provides protein-domain microarray-based protein interaction analysis and determination services to help our global customers conduct relevant research and reduce research costs. With our time-tested capabilities and expertise, we are confident to provide you with quality services to escort your research.
Protein-domain Microarray Technology
Protein domains mediate protein-protein interactions by binding short peptide units in the corresponding ligands, and this peptide recognition module is essential for the assembly of multi-protein complexes. Because of their small size and unique spatial structure, they are the main force of interaction with proteins in cells, so they are good probe candidates for protein microarrays. In addition, the deactivated recombinant protein domain will still retain some binding specificity. Use recombinant proteins or fluorescently labeled peptides as probes to probe such microarrays to identify potential binding partners and determine how post-translational modifications affect specific interactions. Therefore, protein domain arrays have advantages for elucidating the specificity and regulation of signal transduction pathways. The main idea at the beginning of the design of the protein domain chip is to identify the potential binding partners of the protein and identify the quality difference of the protein ligand (caused by post-translational modification).
Fig 1. Protein domain microarrays. (Kaushansky A, et al. 2010)
Service Process
Our protein interaction microarray service relies on domain microarray technology and addressing microarrays to help identify proteins that interact with motifs with post-translational modifications (PTMs). This high-throughput method can quickly identify protein-protein interactions in vitro. At the same time, it can be verified by other in vitro experiments and in vivo experiments. PTM related research can choose our service. Just send us the biological sample or the extracted protein sample, and we will help you complete the subsequent interaction analysis. Our services include unique customized microarray services. These services that have been developed so far contain many unique features that have been identified, enabling them to further characterize these protein-protein interactions in cells. In addition, we will provide instruments and expertise to determine the strength of these protein-protein interactions. The service process includes probe design, expression vector construction, probe generation, array printing, microarray detection and data analysis. You can also choose prefabricated microarray products for testing.
Fig 2. Schematic diagram of the service process.
Technology Benefits
The current services include the following types of microarrays. The domain array we currently provide can be used to analyze the following content.
- Used for lysine acetylation ligand analysis
- Used for methylarginine and lysine ligand analysis
- Used for ubiquitin binding domain analysis
- For free C-terminal ligand analysis
- For proline-rich ligand analysis
- For threonine/serine ligand analysis
- For phosphotyrosine ligand analysis
Creative Proteomics provides high-quality microarray tools and convenient detection services for analyzing protein interactions. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. By choosing outsourcing testing services, your advantage is that you do not need to consider any other expenses, such as equipment, laboratory space, supervision or management. This convenient method can quickly turn around and provide reliable results, thus saving valuable time and resources. Please contact us for more detailed information.
References
- Espejo A, Côté J, Bednarek A, et al. A protein-domain microarray identifies novel protein–protein interactions[J]. Biochemical Journal, 2002, 367(3): 697-702.
- Kaushansky A, Allen J E, Gordus A, et al. Quantifying protein–protein interactions in high throughput using protein domain microarrays[J]. Nature protocols, 2010, 5(4): 773.
For research use only, not intended for any clinical use.
