As a trusted supplier in the field of microarrays, Creative Proteomics is committed to designing and developing various effective biological microarray products and providing corresponding testing services. Our microarray detection platform provides a full range of microarray-based analysis services to help our customers reduce costs while using advanced technologies for related research. We are confident to provide you with high-quality products and high-quality services to accelerate the output of research results.
Histone Microarray
The dynamic regulation of chromatin structure is related to the regulation of all DNA processes including gene expression. The regulation of gene expression and chromatin state through epigenetic modification is an important process of development, physiology and disease. Histone post-translational modification (PTM) is one of the main mechanisms that regulate chromatin structure and activity. It has been reported that there are more than 100 different histone PTMs that have been identified, many of which have clear roles in gene expression regulation and chromatin biology. Histone PTM includes acetylation, phosphorylation, and methylation. These different chemical characteristics affect chromatin structure in a type and site-specific manner. One of the main mechanisms by which histone PTM changes the structure of chromatin is through the recruitment of effector proteins, which contain special "reader" domains that specifically recognize different histone PTMs. These effectors may be transcription factors or additional chromatin modification mechanisms, and their functions usually define downstream results related to different histone PTMs. Microarray technology can simultaneously interrogate thousands of macromolecular interactions (protein-DNA, protein-protein, and protein-peptide) in a high-throughput, reproducible, and miniaturized form.
Fig 1. Cartoon Depiction of the Stepwise Procedure for Antibody Screening on a Histone Peptide microarray. (Cornett E M, et al. 2017)
Service Process
We provide histone modification analysis services and histone antibody screening services based on antibody microarrays and peptide microarrays. Peptide arrays can be used to analyze the specificity of peptide modifying enzymes and the binding specificity of antibodies, as well as proteins including epigenetic reading domains. The histone peptide microarray has become an information discovery platform for chromatin biochemistry research, capable of high-throughput analysis of histone PTM authors, erasers and readers, and also used to analyze histone antibody specificity. Our histone peptide micros contain prefabricated and customized microarrays. The microarray probe sets cover peptides from different regions of the five major core histone families, as well as nearly 70 post-translational modifications in many different combinations. Our histone peptide microarray service can be used to select histone antibodies and analyze protein-protein interactions. The service process includes sample pre-processing, microarray testing and data processing.
Antibodies are key reagents for detecting histone PTM, and histone PTM specific antibodies can be used for chromatin biochemistry research. Our histone antibody microarray is a customized service. The service process includes microarray analysis design, manufacturing, microarray detection, and data analysis.
Fig 2. Our histone microarray service process.
Our Features
- Wide range of applications. Our services can be applied to basic research and antibody development;
- multiple choices. Our customized and semi-customized products meet the individual needs of customers;
Creative Proteomics provides diversified histone microarray tools and convenient detection services. We have a large team of scientists with a solid academic foundation and cooperate with researchers in basic biology and biomedical projects from all over the world. The advantage of choosing an analytical testing outsourcing testing service is that there is no need to consider any other costs (such as equipment and laboratory space), and it saves supervision or management. This advanced technology can quickly turn around and provide reliable results, thereby saving valuable time and resources. Please contact us for more detailed information.
References
- Cornett E M, Dickson B M, Rothbart S B. Analysis of histone antibody specificity with peptide microarrays[J]. Journal of visualized experiments: JoVE, 2017 (126).
- Bock I, Dhayalan A, Kudithipudi S, et al. Detailed specificity analysis of antibodies binding to modified histone tails with peptide arrays[J]. Epigenetics, 2011, 6(2): 256-263.
- Kondo Y, Shen L, Yan P S, et al. Chromatin immunoprecipitation microarrays for identification of genes silenced by histone H3 lysine 9 methylation[J]. Proceedings of the National Academy of Sciences, 2004, 101(19): 7398-7403.
For research use only, not intended for any clinical use.
