As a trusted supplier in the field of microarrays, Creative Proteomics is committed to designing and developing various effective biological microarray products and providing corresponding testing services. Our microarray detection platform provides a full range of microarray-based analysis services to help our customers reduce costs while using advanced technologies for related research. We are confident to provide you with high-quality products and high-quality services to accelerate the output of research results.
Monoclonal Screening and Isolation
Monoclonal antibodies (mAbs) are highly specific biomolecules with great scientific and practical significance. Compared with polyclonal antibodies from immune serum, they are homogeneous and monospecific because they are produced by hybridoma cells that represent a single cell clone. The production of monoclonal antibodies is a multi-stage, time-consuming and expensive process. In the past, monoclonal screening and isolation were mostly time-consuming. The growing demand for these molecules has created room for research focused on improving hybridoma technology. Reducing costs, manpower and time is an important goal of these technological improvements.
Service Process
We provide monoclonal antibody screening microarray services based on peptide microarray and cell array technology. In order to achieve high-throughput single cell separation and analysis, a microarray-based screening method was developed. The protocol is based on a microarray executed on a standard slide format. We can accept antibodies derived from phage display technology or hybridoma cells derived from mice. For samples derived from antibodies screened by phage display technology, our services include single-cell dispersion microarray design, microarray manufacturing, microarray-based FISH detection, data harvesting and analysis. Customers can also submit screenings for hybridoma cells derived from mice. We provide detection methods based on microwell microarrays. The service process includes single cell dispersion (analysis of cell products based on single cells), microarray hybridization (use of fluorescently labeled antigens for detection), data harvesting and analysis. Please consult our experts for details.
Fig 1. Our microarray-based monoclonal screening service process.
Our Features and Benefits
- A variety of screening strategies are available;
- Industry-leading equipment and technology platform;
- An experienced team of experts;
Creative Proteomics provides you with customized monoclonal screening services based on microarrays. We have a large team of scientists with a solid academic foundation and cooperate with researchers in basic biology and biomedical projects from all over the world. The advantage of choosing an analytical testing outsourcing testing service is that there is no need to consider any other costs (such as equipment and laboratory space), and it saves supervision or management. This advanced technology can quickly turn around and provide reliable results, thereby saving valuable time and resources. Please contact us for more detailed information.
References
- Jin A, Ozawa T, Tajiri K, et al. Rapid isolation of antigen-specific antibody-secreting cells using a chip-based immunospot array[J]. Nature protocols, 2011, 6(5): 668-676.
- Kishi H, Jin A, Ozawa T, et al. Screening of antigen-specific antibody-secreting cells[M]//Single-Cell Analysis. Humana Press, 2012: 141-150.
- Kishi H, Ozawa T, Hamana H, et al. Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array[M]//Human Monoclonal Antibodies. Humana Press, New York, NY, 2019: 147-162.
For research use only, not intended for any clinical use.
